OncoBEAM™
Liquid Biopsy Testing

OncoBEAMTM liquid biopsy tests utilize BEAMing technology (Beads, Emulsions, Amplification & Magnetics), which is an ultra-sensitive method specifically designed for circulating tumor DNA (ctDNA) analysis. The BEAMing method employs an optimized digital PCR technique combined with flow cytometry that ensures each molecule of input DNA is analyzed to ensure reliable detection of rare mutant molecules.

OncoBEAMTM was the first established digital PCR technology platform for liquid biopsy and has been used to advance both clinical validity and utility of plasma-based detection of various clinically-relevant mutations across a number of cancer types such as non-small cell lung cancer, colorectal cancer, and estrogen receptor-positive/ HER2-negative breast cancer.

We offer the following OncoBEAMTM assays to support development of targeted therapies, as well as exploration of novel clinical applications for ctDNA such as molecular monitoring of treatment response and minimal/measurable residual disease (MRD). These assays are available for prospective testing in CLIA/GCP environments.

OncoBEAM Tumor Specific Tests

GENES Exons Codon(s) Mutations [Nucleotide Change]
IDH1 4 132 R132S [394C>A], R132G [394C>G], R132C [394C>T], R132H [395G>A], R132L [395G>T]
IDH2 4a 140 R140W [418C>T], R140Q [419G>A], R140L [419G>T]
4b 172 R172K [515G>A], R172M [515G>T]

GENES Exons Codon(s) Mutations [Nucleotide Change]
AKT1 2 17 E17K [49G>A]
ESR1 5 380 E380Q [1138G>C]
7 463 S463P [1387T>C]
8 534, 535, 536, 537, 538 V534E [1601T>A], P535H [1604C>A], L536H [1607T>A], L536P [1607T>C], L536R [1607T>G], L536Q [1607_1608delinsAG], Y537N [1609T>A], Y537S [1610A>C], Y537C [1610A>G], D538G [1613A>G]
PIK3CA 7 420 C420R [1258T>C]
9 542, 545, 546 E542K [1624G>A], E545G [1634A>G], E545K [1633G>A], Q546K [1636C>A]
20 1043, 1047 M1043I [3129G>T], H1047R [3140A>G], H1047L [3140A>T], H1047Y [3139C>T]

Additional mutations are available in PIK3CA(10,21)/AKT1(2)/ BRAF(15). For detailed information contact your local key Account Manager or drop us a line via info@sysmex-inostics.com

GENES Exons Codon(s) Mutations [Nucleotide Change]
KRAS 2 12,13 G12S [34G>A], G12R [34G>C], G12C [34G>T], G12D [35G>A], G12A [35G>C], G12V [35G>T], G13D [38G>A]
3 59, 61 A59T [175G>A], Q61H [183A>C, 183A>T], Q61L [182A>T]
4 117, 146 K117N [351A>C, 351A>T], A146T [436G>A], A146V [437C>T]
NRAS 2 12,13 G12S [34G>A], G12R [34G>C], G12C [34G>T], G12D [35G>A], G12A [35G>C], G12V [35G>T], G13R [37G>C], G13D [38G>A], G13V [38G>T]
3 59, 61 A59T [175G>A], Q61H [183A>C, 183A>T], Q61R [182A>G], Q61L [182A>T], Q61K [181C>A]
4 117, 146 K117N [351C>A, 351A>T], A146T [436G>A]
BRAF 15 600 V600E [1799T>A]
Genes Exons Codon(s) Mutations [Nucleotide Change]
PIK3CA 10 542, 545, 546 E542K [1624G>A],E545G [1633G>A], E545K [1634A>G], Q546K [1636C>A]
21 1043, 1047 M1043I [3129G>T], H1047R [3140A>G], H1047L[3140A>T], H1047Y[3139C>T]
AKT1 2 17 E17K [49G>A]
BRAF 15 600 V600K [1798_1799delinsAA]

GENES Exons Codon(s) Mutations [Nucleotide Change]
IDH1 4 132 R132S [394C>A], R132G [394C>G], R132C [394C>T], R132H [395G>A], R132L [395G>T]
IDH2 4a 140 R140W [418C>T], R140Q [419G>A], R140L [419G>T]
4b 172 R172K [515G>A], R172M [515G>T]

GENES Exons Codon(s) Mutations [Nucleotide Change]
BRAF 15 600 V600E [1799T>A]
EGFR 19 746, 747 E746_A750del [2235_2249del, 2236_2250del], E746_S752>V [2237_2255delinsT], L747_A750>P [2239_2248delinsC], L747_T751del [2240_2254del], L747_P753>S [2240_2257del]
20 790, 797 T790M [2369C>T], C797S [2389T>A, 2390G>C]
21 858, 861 L858R [2573T>G], L861Q [2582T>A]
KRAS 2 12, 13 G12S [34G>A], G12R [34G>C], G12C [34G>T], G12D[35G>A], G12A [35G>C], G12V [35G>T], G13D [38G>A]
3 61 Q61L [182A>T], Q61H [183A>T, 183A>C]

Genes Exons Codon(s) Mutations [Nucleotide Change]
BRAF 15 600 V600E [1799T>A], V600K [1798_1799delinsAA]
NRAS 3 61 Q61H [183A>C, 183A>T], Q61R [182A>G], Q61L [182A>T], Q61K [181C>A]

Genes Exons Codon(s) Mutations [Nucleotide Change]
KRAS 2 12,13 G12S [34G>A], G12R [34G>C], G12C [34G>T], G12D [35G>A], G12A [35G>C], G12V [35G>T], G13D [38G>A]
3 59, 61 A59T [175G>A], Q61H [183A>C, 183A>T], Q61L [182A>T]
4 117, 146 K117N [351A>C, 351A>T], A146T [436G>A], A146V [437G>T]
NRAS 2 12,13 G12S [34G>A], G12R [34G>C], G12C [34G>T], G12D [35G>A], G12A [35G>C], G12V [35G>T], G13R [37G>C], G13D [38G>A], G13V [38G>T]
3 59, 61 A59T [175G>A], Q61H [183A>C, 183A>T], Q61R [182A>G], Q61L [182A>T], Q61K [181C>A]
4 117, 146 K117N [351C>A, 351A>T], A146T [436G>A]

GENES Exons Codon(s) Mutations [Nucleotide Change]
AR 4 702, 716 L702H [2105T>A], L702Q [2104_2106delinsACC, 2104_2106delinsACG, 2104_2106delinsACT, 2104_2106delinsACA], V716M [2148G>A]
5 742 W742L [2225G>T], W742C [2226G>T]
8 875, 877, 878, 896 H875Y [2623C>T], F877L [2629T>C, 2631C>A, 2631C>G], T878A [2632A>G], T878S [2633C>G], M896V [2686A>G], M896T [2687T>C]

OncoBEAM Single Gene Tests

Exons Codon(s) Mutations [Nucleotide Change]
2 17 E17K [49G>A]

Exons Codon(s) Mutations [Nucleotide Change]
22 1151, 1152, 1156 T1151dup [3451_3453dup], L1152R [3455T>G], C1156Y [3467G>A]
23 1171 I1171N [3512T>A, 3512_3213delinsAT], I1171T[3512T>C], I1171S [3512T>G]
24a 1174, 1180 F1174C [3521T>G], F1174L [3522C>A], V1180L [3538G>C]
24b 1196, 1198, 1202, 1203, 1206 L1196M [3586C>A], L1198F [3592C>T], G1202R [3604G>A], D1203N [3607G>A], S1206Y [3617C>A]
25 1296, 1275 G1269A [3809G>C], R1275Q [3824G>A]

Exons Codon(s) Mutations [Nucleotide Change]
4 702, 716 L702H [2105T>A], L702Q [2104_2106delinsACC, 2104_2106delinsACG, 2104_2106delinsACT, 2104_2106delinsACA], V716M [2148G>A]
5 742 W742L [2225G>T], W742C [2226G>T]
8 875, 877, 878, 896 H875Y [2623C>T], F877L [2629T>C, 2631C>A, 2631C>G], T878A [2632A>G], T878S [2633C>G], M896V [2686A>G], M896T [2687T>C]

Exons Codon(s) Mutations [Nucleotide Change]
15 600 V600E [1799T>A], V600K [1798_1799delinsAA]

Exons Codon(s) Mutations [Nucleotide Change]
19 746, 747 E746_A750del [2235_2249del, 2236_2250del], E746_S752>V [2237_2255delinsT], L747_A750>P [2239_2248delinsC], L747_T751del [2240_2254del], L747_P753>S [2240_2257del]
20 790, 797 T790M [2369C>T], C797S [2389T>A, 2390G>C]
21 858, 861 L858R [2573T>G], L861Q [2582T>A]

Exons Codon(s) Mutations [Nucleotide Change]
5 380 E380Q [1138G>C]
7 463 S463P [1387T>C]
8 534, 535, 536, 537, 538 V534E [1601T>A], P535H [1604C>A], L536H [1607T>A], L536P [1607T>C], L536R [1607T>G], L536Q [1607_1608delinsAG], Y537N [1609T>A], Y537S [1610A>C], Y537C [1610A>G], D538G [1613A>G]

Exons Codon(s) Mutations [Nucleotide Change]
4 132 R132S [394C>A], R132G [394C>G], R132C [394C>T], R132H [395G>A], R132L [395G>T]

Exons Codon(s) Mutations [Nucleotide Change]
4a 140 R140W [418C>T], R140Q [419G>A], R140L [419G>T]
4b 172 R172K [515G>A], R172M [515G>T]

Exons Codon(s) Mutations [Nucleotide Change]
2 12, 13 G12S [34G>A], G12R [34G>C], G12C [34G>T], G12D [35G>A], G12A [35G>C], G12V [35G>T], G13D [38G>A]
3 59, 61 A59T [175G>A], Q61H [183A>C, 183A>T], Q61L [182A>T]
4 117, 146 K117N [351A>C, 351A>T], A146T [436G>A], A146V [437C>T]

Exons Codon(s) Mutations [Nucleotide Change]
2 12, 13 G12S [34G>A], G12R [34G>C], G12C [34G>T], G12D [35G>A], G12A [35G>C], G12V [35G>T], G13R [37G>C], G13D [38G>A], G13V [38G>T]
3 59, 61 A59T [175G>A], Q61H [183A>C, 183A>T], Q61R [182A>G], Q61L [182A>T], Q61K [181C>A]
4 117, 146 K117N [351C>A, 351A>T], A146T [436G>A]

Exons Codon(s) Mutations [Nucleotide Change]
7 420 C420R [1258T>C]
9 542, 545, 546 E542K [1624G>A], E545G [1634A>G], E545K [1633G>A], Q546K [1636C>A]
20 1043, 1047 M1043I [3129G>T], H1047R [3140A>G], H1047L [3140A>T], H1047Y [3139C>T]

Exons Codon(s) Mutations [Nucleotide Change]
38 2032 G2032R [6094G>A]
40 2092 Y2092C [6275A>G]
41 2155 L2155S [6464T>C]

OncoBEAM Multigene Tests

Genes Exons Codon(s) Mutations [Nucleotide Change]
AKT1 2 17 E17K [49G>A]
ESR1 5 380 E380Q [1138G>C]
7 463 S463P [1387T>C]
8 534, 535, 536, 537, 538 V534E [1601T>A], P535H [1604C>A], L536H [1607T>A], L536P [1607T>C], L536R [1607T>G], L536Q [1607_1608delinsAG], Y537N [1609T>A], Y537S [1610A>C], Y537C [1610A>G], D538G [1613A>G]
PIK3CA 7 420 C420R [1258T>C]
9 542, 545, 546 E542K [1624G>A], E545G [1634A>G], E545K [1633G>A], Q546K [1636C>A]
20 1043, 1047 M1043I [3129G>T], H1047R [3140A>G], H1047L [3140A>T], H1047Y [3139C>T]

Genes Exons Codon(s) Mutations [Nucleotide Change]
ALK 22 1151, 1152, 1156 T1151dup [3451_3453dup], L1152R [3455T>G], C1156Y [3467G>A]
23 1171 I1171N [3512T>A, 3512_3213delinsAT], I1171T[3512T>C], I1171S [3512T>G]
24a 1174, 1180 F1174C [3521T>G], F1174L [3522C>A], V1180L [3538G>C]
24b 1196, 1198, 1202, 1203, 1206 L1196M [3586C>A], L1198F [3592C>T], G1202R [3604G>A], D1203N [3607G>A], S1206Y [3617C>A]
25 1296, 1275 G1269A [3809G>C], R1275Q [3824G>A]
ROS1 38 2032 G2032R [6094G>A]
40 2092 Y2092C [6275A>G]
41 2155 L2155S [6464T>C]

Genes Exons Codon(s) Mutations [Nucleotide Change]
BRAF 15 600 V600E [1799A>T]
EGFR 19 746, 747 E746_A750del [2235_2249del, 2236_2250del], E746_S752>V [2237_2255delinsT], L747_A750>P [2239_2248delinsC], L747_T751del [2240_2254del], L747_P753>S [2240_2257del]
20 790, 797 T790M [2369C>T], C797S [2389T>A, 2390G>C]
21 858, 861 L858R [2573T>G], L861Q [2582T>A]
KRAS 2 12, 13 G12S [34G>A], G12R [34G>C], G12C [34G>T], G12D[35G>A], G12A [35G>C], G12V [35G>T], G13D [38G>A]
3 61 Q61L [182A>T], Q61H [183A>T, 183A>C]

This OncoBEAM Multigene Test is also available as KRAS – NRAS only. For more information contact your local Key Account Manager or drop us a line via info@sysmex-inostics.com

Genes Exons Codon(s) Mutations [Nucleotide Change]
BRAF 15 600 V600E [1799T>A]
KRAS 2 12,13 G12S [34G>A], G12R [34G>C], G12C [34G>T], G12D [35G>A], G12A [35G>C], G12V [35G>T], G13D [38G>A]
3 59, 61 A59T [175G>A], Q61R [182A>G], Q61K [181C>A]
4 117, 146 K117N [351A>C, 351A>T],A146T [436G>A], A146V [437C>T]
NRAS 2 12,13 G12S [34G>A], G12R [34G>C], G12C [34G>T], G12D [35G>A], G12A [35G>C], G12V [35G>T], G13R [37G>C], G13D [38G>A], G13V [38G>T]
3 59, 61 A59T [175G>A], Q61H [183A>C, 183A>T], Q61L [182A>T]
4 117, 146 K117N [351C>A, 351A>T], A146T [436C>A]

Genes Exons Codon(s) Mutations [Nucleotide Change]
BRAF 15 600 V600E [1799T>A]
NRAS 3 61 Q61H [183A>C, 183A>T], Q61R [182A>G], Q61L [182A>T], Q61K [181C>A]

Genes Exons Codon(s) Mutations [Nucleotide Change]
IDH1 4 132 R132S [394C>A], R132G [394C>G], R132C [394C>T], R132H [395G>A], R132L [395G>T]
IDH2 4a 140 R140W [418C>T], R140Q [419G>A], R140L [419G>T]
4b 172 R172K [515G>A], R172M [515G>T]
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